monitoring three plasmopara halstedii resistance genes in iranian sunflower inbred lines

نویسندگان

masood soltani

raha abedini

hassan eskandari

rahim mehrabi

چکیده

background: downy mildew caused by plasmopara halstedii is one of the most devastating diseases of sunflower worldwide. so far several dominant resistance genes designated as pl have been identified and their molecular markers linked to these genes are available. however, no information on the resistance genes is available in iranian lines. objective: in this study, the presence of three map-based molecular markers previously proved to be linked to different resistance genes were evaluated in different sunflower inbred lines. materials and methods: using pcr-based and caps molecular markers, 26 sunflower inbred lines with different responses to p. halstedii race 100 were used to detect the presence of three resistance loci including pl1, pl6 and pl13 within the lines. results: molecular marker linked to pl13 was present in some of the sunflower lines but was not correlated with the phenotypic reaction of the lines to race 100. although three markers linked to pl6 were used, no pcr amplification was obtained indicating that none of the genotypes possessed pl6 locus. pl1-linked caps molecular marker w background: downy mildew caused by plasmopara halstedii is a devastating disease in sunflower worldwide. several dominant resistance genes designated as pl have been identified and linked molecular markers have been demonstrated. however, no information on theresistance genes is available foriranian lines. objectives: the presence of three map-based molecular markers previously proved to be linked to different resistance genes were evaluated in sunflower inbred lines. materials and methods: using pcr-based and caps molecular markers, 26 sunflower inbred lines with different responses to p. halstedii race 100 were used to detect the presence of three resistance loci including pl1, pl6 and pl13 within the lines. results: molecular marker linked to pl13 was present in some of the sunflower lines but was not correlated with the phenotypic reaction of the lines to race 100. despite the use of three markers linked to pl6, pcr failed to amplify any corresponding product. this data may suggest that none of the genotypes possessed pl6 locus. pl1-linked cleaved amplified polymorphic sequences (caps) were present in several resistance lines and effectively differentiated susceptible and resistant sunflower lines. conclusions: applicability of molecular markers in breeding programs revisited in disease management.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Monitoring Three Plasmopara halstedii Resistance Genes in Iranian Sunflower Inbred Lines

Background: Downy mildew caused by Plasmopara halstedii is one of the most devastating diseases of sunflower worldwide. So far several dominant resistance genes designated as Pl have been identified and their molecular markers linked to these genes are available. However, no information on the resistance genes is available in Iranian lines. Objective: In this study, the presence of three map-b...

متن کامل

Components of Quantitative Resistance to Downy Mildew (plasmopara Halstedii) in Sunflower (helianthus Annuus)

Components of quantitative resistance in sunflower (Helianthus annuus) to Plasmopara halstedii, the pathogen causing downy mildew, were investigated. Percentage infection, latent period, sporulation density and reduction of hypocotyl length were compared on two sunflower lines showing different levels of quantitative resistance in the field infected with different P. halstedii strains of races ...

متن کامل

RXLR and CRN Effectors from the Sunflower Downy Mildew Pathogen Plasmopara halstedii Induce Hypersensitive-Like Responses in Resistant Sunflower Lines

Plasmopara halstedii is an obligate biotrophic oomycete causing downy mildew disease on sunflower, Helianthus annuus, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. Pl resistance genes conferring resistance to specific P. halstedii pathotypes were located on sun...

متن کامل

Induction of a sunflower CC-NBS-LRR resistance gene analogue during incompatible interaction with Plasmopara halstedii.

Downy mildew caused by Plasmopara halstedii is one of the main diseases causing economic losses in cultivated sunflower. Resistance in this host is conferred by major genes denoted Pl. The inbred sunflower line QIR8, which contains the Pl8 locus and is resistant to all known downy mildew races, was used to isolate a full-length resistance gene analogue (RGA) belonging to the CC-NBC-LRR class of...

متن کامل

Impact of major gene resistance management for sunflower on fitness of Plasmopara halstedii \(downy mildew\) populations

1 Inra-UBP, UMR 1095, 234, avenue du Brézet, 63100 Clermont-Ferrand, France 2 Inra-CNRS, UMR 441, BP 52627, 31326 Castanet-Tolosan, France 3 Inra-UBP, UMR 1095, 24, avenue des Landais, 63177 Clermont-Ferrand, France 4 CETIOM, ENSAT, 18 Chemin de Borde-Rouge, BP 32607 31326 Auzeville-Tolosane France 5 Inra, UMR santé végétale (Inra-Enita), centre de recherches de Bordeaux, la Grande Ferrade, BP ...

متن کامل

Molecular characterization of two types of resistance in sunflower to Plasmopara halstedii, the causal agent of downy mildew.

Depending on host-pathotype combination, two types of sunflower-Plasmopara halstedii incompatibility reactions have previously been identified. Type I resistance can restrict the growth of the pathogen in the basal region of the hypocotyls, whereas type II cannot, thus allowing the pathogen to reach the cotyledons. In type II resistance, a large portion of the hypocotyls is invaded by the patho...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of biotechnology

ناشر: national institute of genetic engineering and biotechnology

ISSN 1728-3043

دوره 13

شماره 2 2015

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023